pET23a载体基本信息
出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列:  载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
EMD Biosciences(Novagen)
pET23a, pet 23a
大肠杆菌蛋白表达
高
多克隆位点，限制性内切酶
3666bp
T7
5'-TAATACGACTCACTATAGGG-3'
N-T7, C-His
Ampicillin
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
 69745-3
瞬时表达 Transient
组成型 Constitutive
非病毒
pET23a载体质粒图谱和多克隆位点信息


Feature Name 
Start 
End 
 
T7 Promoter
303
319
T7 Transcription start
302
302
T7•Tag coding sequence
239
207
Multiple cloning sites
158
203
His•Tag coding sequence
157
140
T7 terminator
26
72
pBR322 origin
1450
1450
bla coding sequence
2211
3068
f1 origin
3200
3655
pET23a载体简介
The pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) by the “plain” T7 promoter instead of the T7lac promoter and by the absence of the lacI gene. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
pET23a载体序列
LOCUS       pET-23a(+)              3666 bp    DNA     circular     11-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     promoter        complement(303..319)
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    complement(207..239)
                     /vntifkey="31"
                     /label=T7\tag
     protein_bind    complement(140..157)
                     /vntifkey="31"
                     /label=His\tag
     terminator      complement(26..72)
                     /vntifkey="43"
                     /label=T7\terminator
     rep_origin      complement(1450..1450)
                     /vntifkey="33"
                     /label=ColE1\pBR322\origin
     CDS             complement(2211..3068)
                     /vntifkey="4"
                     /label=bla\(Ap)\sequence
     rep_origin      complement(3200..3655)
                     /vntifkey="33"
                     /label=f1\origin
BASE COUNT      919 a       936 c       926 g       885 t
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata
      241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc
      301 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc
      361 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
      421 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
      481 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
      541 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
      601 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
      661 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
      721 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
      781 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
      841 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
      901 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
      961 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     1141 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     1201 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1261 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
TUNEology 波长可调检测卡盒-->